3B37
Crystal structure of E. coli Aminopeptidase N in complex with Tyrosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.324, 120.324, 170.345 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.880 - 1.700 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.18400 |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.207 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 156411 | |
<I/σ(I)> | 32.24 | 2.9 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 5.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M Sodium Malonate, pH=7.0, vapor diffusion, hanging drop, temperature 298K |