3B0B

Crystal structure of the chicken CENP-S/CENP-X complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9642, 0.97944, 0.97889, 0.9951
Spacegroup name	P 61
Unit cell lengths	48.741, 48.741, 345.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.211 - 2.150
R-factor	0.1901
Rwork	0.186
R-free	0.23480
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.959
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.211	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	25152
Completeness [%]	99.7	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	100mM Citrate-BisPropane pH 9.5, 500mM NaCl, 100mM MgSO4, and 30% PEG 600, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	9.5	293	100mM Citrate-BisPropane pH 9.5, 500mM NaCl, 100mM MgSO4, and 30% PEG 600, VAPOR DIFFUSION, temperature 293K