3ASI
Alpha-Neurexin-1 ectodomain fragment; LNS5-EGF3-LNS6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.910, 79.380, 78.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.070 - 2.300 |
R-factor | 0.1997 |
Rwork | 0.197 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c4r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.124 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.070 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.083 | 0.271 |
Number of reflections | 20732 | |
<I/σ(I)> | 20.9 | 6.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG3000, 0.1M sodium acetate and 0.3M sodium malonate pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293KK |