3APZ
Apo form of Arabidopsis medium/long-chain length prenyl pyrophosphate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-27 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 148.512, 150.126, 176.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
Rwork | 0.206 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wy0 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.071 | 0.489 |
Number of reflections | 33211 | |
<I/σ(I)> | 18.3 | 2.5 |
Completeness [%] | 99.5 | 98.8 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 295 | 60%(v/v) Tacsimate, pH 7.0, vapor diffusion, temperature 295K |