3AMV
ALLOSTERIC INHIBITION OF GLYCOGEN PHOSPHORYLASE A BY A POTENTIAL ANTIDIABETIC DRUG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07 |
Detector | MAR scanner 180 mm plate |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 127.200, 127.200, 115.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.000 - 2.100 |
Rwork | 0.189 |
R-free | 0.26000 |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.088 | 0.242 |
Total number of observations | 337366 * | |
Number of reflections | 51612 | |
<I/σ(I)> | 11.35 | 2.5 |
Completeness [%] | 92.9 | 87.3 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.7 | 100 * | 1 MM W1807 IN A MEDIUM CONSISTING OF 20-24 MG/ML ENZYME, 10 MM MAGNESIUM ACETATE, 3 MM DTT, 10 MM BES, 0.1 MM EDTA, AND 0.02% SODIUM AZIDE, PH 6.7 (22 DEG C). |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Glc | 50 (mM) | |
2 | 1 | 1 | W1807 | 1 (mM) |