3AJY
Crystal Structure of Ancestral Congerin Con-anc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-07 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.777, 53.885, 132.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.850 - 2.010 |
R-factor | 0.20801 |
Rwork | 0.206 |
R-free | 0.24687 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c1f |
RMSD bond length | 0.011 |
RMSD bond angle | 1.435 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 20146 | |
<I/σ(I)> | 42.8 | 6.7 |
Completeness [%] | 98.2 | 89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.51 | 293 | 0. 1M MMT, 25%(w/v) PEG1500, 1mM lactose, pH 6.51, VAPOR DIFFUSION, HANGING DROP, temperature 293K |