3AGY
Crystal structure of human Hsp40 Hdj1 peptide-binding domain complexed with a C-terminal peptide of Hsp70
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9780 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.746, 41.132, 62.865 |
Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
Resolution | 42.710 - 1.850 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.435 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.700 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.056 | 0.482 |
Number of reflections | 45431 | |
<I/σ(I)> | 23.1 | 1.5 |
Completeness [%] | 99.1 | 96.9 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 18% PEG 3350, 0.1M Na citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |