Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-21 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.809, 69.079, 65.642 |
| Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
| Resolution | 28.960 - 1.700 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hdh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.075 | 0.537 |
| Number of reflections | 34467 | |
| <I/σ(I)> | 9 | 3.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 291 | ammonium acetate, sodium acetate trihydrate, polyethylene glycol 4000, glycerol anhydrous, pH 4.6, MICROBATCH, temperature 291K |
