3A32
Crystal structure of putative threonyl-tRNA synthetase ThrRS-1 from Aeropyrum pernix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.486, 103.710, 112.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.300 - 2.300 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a31 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | 0.300 |
| Number of reflections | 21565 | |
| <I/σ(I)> | 19.9 | 6.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5M ammonium sulfate in 0.1M HEPES (pH7.5) buffer, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
