377D
5'-R(*CP*GP*UP*AP*CP*DG)-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS II |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 29.530, 44.610, 94.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.760 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.23200 |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.400 * |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 * | |
High resolution limit [Å] | 1.760 * | 1.760 * |
Rmerge | 0.067 * | |
Number of reflections | 4757 * | |
Completeness [%] | 76.0 * | 65 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | 291 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 2 (mM) | single strand concentration |
2 | 1 | drop | magnesium acetate | 2.5 (mM) | |
3 | 1 | drop | spermine | 0.5 (mM) | |
4 | 1 | drop | sodium cacodylate | 10 (mM) | |
5 | 1 | drop | DMA | 0.2 (mM) | |
6 | 1 | reservoir | MPD | 50 (%) |