2ZGW
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A
Replaces: 2E3YExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.308, 82.876, 72.578 |
Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
Resolution | 37.250 - 1.500 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.20400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1wqw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.440 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.042 | 0.274 |
Number of reflections | 69128 | |
<I/σ(I)> | 12.8 | 2.1 |
Completeness [%] | 98.0 | 95.6 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.2 | 295 | PEG20K, Acetate, NaOH, ATP, Biotin, pH 5.2, microbatch, temperature 295K |