2ZGV
Crystal Structure of human phosphoglycerate kinase bound to D-ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2007-05-22 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.956, 106.627, 50.350 |
Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
Resolution | 45.220 - 2.000 |
R-factor | 0.17782 |
Rwork | 0.176 |
R-free | 0.25828 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vjc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.697 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.220 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.048 | |
Number of reflections | 16987 | |
<I/σ(I)> | 11.3 | 2.9 |
Completeness [%] | 0.7 | 0.31 |
Redundancy | 2.6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.6M NaKPO4, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |