2Z2S
Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2003-02-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97969 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.135, 46.452, 141.701 |
Unit cell angles | 90.00, 91.63, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.700 |
R-factor | 0.2865 |
Rwork | 0.250 |
R-free | 0.28650 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.079 | 0.079 |
Number of reflections | 24975 | |
<I/σ(I)> | 9.8 | 2.14 |
Completeness [%] | 75.8 | 60.7 |
Redundancy | 2.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.15 | 0.1 M MES, 0.2 M ammonium sulfate, 14-18% polyethylene glycol monomethylether 5000 (PEG5KMME), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |