2YWW
Crystal structure of aspartate carbamoyltransferase regulatory chain from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-29 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.282150, 1.20000, 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.003, 71.398, 92.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.960 - 2.000 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.22600 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 3.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.211 |
Number of reflections | 23638 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.16M Ammmounium Acetate, 0.08M Bis-Tris(pH5.5), 20%w/v PEG3350, 10mM ATP, 10mM Zinc chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |