2YO1
Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9- cfII)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-19 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.030, 48.780, 135.830 |
Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
Resolution | 38.640 - 3.100 |
R-factor | 0.26752 |
Rwork | 0.265 |
R-free | 0.32049 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcm |
RMSD bond length | 0.014 |
RMSD bond angle | 1.324 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.100 | 3.280 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.100 | 0.420 |
Number of reflections | 18792 | |
<I/σ(I)> | 8.93 | 2.35 |
Completeness [%] | 96.9 | 94.6 |
Redundancy | 2.31 | 2.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 15 % (V/V) BUTANEDIOL, 100 MM SODIUM ACETATE PH 4.2 |