2YMW
Structure of the epsilon-lysine oxidase from Marinomonas mediterranea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-20 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 70.680, 127.883, 106.897 |
Unit cell angles | 90.00, 107.18, 90.00 |
Refinement procedure
Resolution | 35.280 - 2.410 |
R-factor | 0.1963 |
Rwork | 0.192 |
R-free | 0.27920 |
Structure solution method | MIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.280 | 2.540 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.110 | 0.660 |
Number of reflections | 68875 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 98.8 | 94 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 10% ETHANOL, 0.2 M MAGNESIUM CHLORIDE, 0.1 M IMIDAZOLE 8.0 |