2YML
Native L-haloacid dehalogenase from a Rhodobacteraceae family bacterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.840, 68.340, 159.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 1.790 |
| R-factor | 0.15981 |
| Rwork | 0.157 |
| R-free | 0.20408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2no4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.448 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.080 | 0.730 |
| Number of reflections | 46369 | |
| <I/σ(I)> | 15.1 | 2.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
