2YH9
Crystal structure of the dimeric BamE from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.660, 96.520, 50.490 |
Unit cell angles | 90.00, 134.06, 90.00 |
Refinement procedure
Resolution | 22.290 - 1.800 |
R-factor | 0.18366 |
Rwork | 0.181 |
R-free | 0.22859 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.025 |
RMSD bond angle | 1.831 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.470 |
Number of reflections | 21093 | |
<I/σ(I)> | 25.7 | 2.8 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5 |