2YGT

Clostridium perfringens delta-toxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-05
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	112.932, 49.664, 58.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.477 - 2.400
R-factor	0.1812
Rwork	0.179
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pvl
RMSD bond length	0.008
RMSD bond angle	0.772
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.200	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.150	0.690
Number of reflections	13432
<I/σ(I)>	15.8	3.5
Completeness [%]	99.9	99.8
Redundancy	10.4	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	293	6.6 MG/ML PROTEIN AGAINST 25-30% PEG 550 25 MM ZNSO4, 100 MM MES-NAOH PH 6.0, AT 293K.