2YEP
STRUCTURE OF AN N-TERMINAL NUCLEOPHILE (NTN) HYDROLASE, OAT2, IN COMPLEX WITH GLUTAMATE
Replaces: 2W4NExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | OXFORD DIFFRACTION |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.220, 73.424, 172.327 |
| Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
| Resolution | 61.120 - 2.700 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vz6 CHAIN A |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.120 | |
| High resolution limit [Å] | 2.700 | |
| Rmerge | 0.100 | |
| Number of reflections | 60743 | |
| Completeness [%] | 96.0 | 95.7 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 290 | 1.4M AMMONIUM SULPHATE, 100MM N-ACETYL -L-GLUTAMATE, 200MM NACL, 100MM TRIS HCL PH 7.5 . |
