2Y8Y
Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.280, 71.210, 76.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.752 - 1.440 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.20540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y8w |
RMSD bond length | 0.006 |
RMSD bond angle | 1.043 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.480 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.050 | 0.850 |
Number of reflections | 44574 | |
<I/σ(I)> | 22.56 | 2.29 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.9 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 0.1 M SODIUM ACETATE, PH 5.0 4% PEG (W/V) 6000. |