2Y0O
The structure of a D-lyxose isomerase from the sigmaB regulon of Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.716, 42.619, 65.881 |
| Unit cell angles | 90.00, 121.30, 90.00 |
Refinement procedure
| Resolution | 34.872 - 1.229 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.17820 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.559 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.450 | 1.300 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 51297 | |
| <I/σ(I)> | 13.8 | 2.6 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 8.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM CACODYLATE PH 6.5 0.2 M NACL 2.0 M AMMONIUM SULFATE 1UL:1UL DROPS WITH PROTEIN IN WATER AT 11.5 MG/ML |
