2XY7
Crystal structure of a salicylic aldehyde base in the pre-insertion site of fragment DNA polymerase I from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.037, 94.022, 106.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.020 - 3.050 |
| R-factor | 0.23853 |
| Rwork | 0.236 |
| R-free | 0.28373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u45 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.150 | 0.530 |
| Number of reflections | 16829 | |
| <I/σ(I)> | 9.5 | 3 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
