2XM0
Cytochrome c prime from Alcaligenes xylosoxidans: Ferrous R124K variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.635, 53.635, 181.285 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.450 - 1.250 |
| R-factor | 0.20317 |
| Rwork | 0.202 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.258 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.300 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.080 | 0.930 |
| Number of reflections | 43230 | |
| <I/σ(I)> | 34.2 | 2.7 |
| Completeness [%] | 98.5 | 96.6 |
| Redundancy | 16.3 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, HEPES PH7.5 |
