2XCD
Structure of YncF,the genomic dUTPase from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-19 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.810, 98.840, 99.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.840 |
R-factor | 0.156 |
Rwork | 0.155 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2baz |
RMSD bond length | 0.013 |
RMSD bond angle | 1.424 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.330 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.070 | 0.880 |
Number of reflections | 81408 | |
<I/σ(I)> | 12.8 | 1.5 |
Completeness [%] | 97.9 | 99.2 |
Redundancy | 5.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2M MGCL2,28% PEG 550, 0.1M HEPES PH 7.5 |