2X3W
structure of mouse syndapin I (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.280, 154.610, 191.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.360 - 2.640 |
| R-factor | 0.22335 |
| Rwork | 0.221 |
| R-free | 0.27373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.350 | 2.750 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 38766 | |
| <I/σ(I)> | 10.81 | 2.88 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 7.17 | 7.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 2.5UL SYNDAPIN(5MG/ML IN 50MM HEPES, 50MM NACL) MIXED WITH 0.5UL 0.3M GLYCYL-GLYCYL-GLYCINE AND 2UL WELL SOLUTION 0.1M NAAC/HAC PH5.0, 3%(W/V) PEG4000., pH 5.3 |
