2X2S
Crystal structure of Sclerotinia sclerotiorum agglutinin SSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 127.318, 127.318, 86.652 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 110.430 - 1.600 |
| R-factor | 0.1409 |
| Rwork | 0.140 |
| R-free | 0.16197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL GENERATED BY PHYRE SERVER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.506 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 100976 | |
| <I/σ(I)> | 16.8 | 2.6 |
| Completeness [%] | 96.2 | 80.2 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M SODIUM CHLORIDE, 0.1 M SODIUM ACETATE, PH 4.6 |
