2WZ1
STRUCTURE OF THE CATALYTIC DOMAIN OF HUMAN SOLUBLE GUANYLATE CYCLASE 1 BETA 3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2009-10-14 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.590, 90.380, 140.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.000 - 1.630 |
R-factor | 0.18536 |
Rwork | 0.184 |
R-free | 0.21922 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3et6 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.585 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 1.670 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 49220 | |
<I/σ(I)> | 9.9 | 2.1 |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PROTEIN WAS CONCENTRATED TO 12 MG/ML IN PURIFICATION BUFFER (10MM HEPES, 500MM NACL, 5% GLYCEROL, 10% GALACTOSE, 0.5MM TCEP)AND SET UP IN SITTING DROP IN A 1:2 RATIO WITH HAMPTON INDEX SCREEN - B8 (1.4M TRI-SODIUM CITRATE DIHYDRATE, 0.1M HEPES, PH 7.5). |