2WX9
Crystal structure of the sialic acid binding periplasmic protein SiaP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.730, 74.490, 87.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.250 - 1.370 |
| R-factor | 0.118 |
| Rwork | 0.116 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4c |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.681 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.250 | 1.450 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.080 | 0.620 |
| Number of reflections | 62379 | |
| <I/σ(I)> | 12.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 36 MG/ML SIAP (IN 20 MM HEPES, 10 MM NACL,AND NEU5GC PH 8.0) AND EQUAL VOLUME OF RESERVOIR SOLUTION (100MM MES PH 6.0, 28.5% PEG 6K, 3% W/V 1,5-DIAMINOPENTANE DIHYDROCHLORIDE) TEMPERATURE 277K |
