2WRY
Crystal structure of chicken cytokine interleukin 1 beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2008-09-21 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.739, 38.476, 102.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.580 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9ilb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.536 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CCP4I |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.050 | 0.560 |
Number of reflections | 20047 | |
<I/σ(I)> | 31.54 | 3.56 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | PH 10 |