2WPZ
GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 25.380, 25.440, 33.700 |
Unit cell angles | 83.98, 84.86, 78.55 |
Refinement procedure
Resolution | 19.510 - 1.250 |
R-factor | 0.18126 |
Rwork | 0.178 |
R-free | 0.22339 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.369 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.330 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.040 | 0.470 |
Number of reflections | 20616 | |
<I/σ(I)> | 10.8 | 1.85 |
Completeness [%] | 91.1 | 87.4 |
Redundancy | 2 | 1.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEG 4000, 200 MM NA-ACETATE, 0.1 M TRIS, PH 8.5 |