2WMO
Structure of the complex between DOCK9 and Cdc42.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-17 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.060, 88.590, 89.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.990 - 2.200 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wm9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.051 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.490 |
Number of reflections | 38312 | |
<I/σ(I)> | 11.9 | 3 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.0 |