2WGZ
Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-05 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.146, 94.222, 94.465 |
Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
Resolution | 25.290 - 2.120 |
R-factor | 0.16959 |
Rwork | 0.169 |
R-free | 0.20582 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4v |
RMSD bond length | 0.007 |
RMSD bond angle | 1.001 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.090 | 0.220 |
Number of reflections | 44578 | |
<I/σ(I)> | 12.4 | 5.14 |
Completeness [%] | 94.4 | 80.7 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 5% PEG 6000, 0.1M TRIS-HCL, PH 8.0 IN THE PRESENCE OF 10MM MNCL2 AND 10MM UDP |