2WCS
Crystal Structure of Debranching enzyme from Nostoc punctiforme (NPDE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 95 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.731, 93.731, 257.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.880 - 2.800 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wc7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.560 |
Number of reflections | 16274 | |
<I/σ(I)> | 11.3 | 7.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 22.8 | 23.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 20%(W/V)POLYETHYLENE GYLCOL, 200MM TRIC-CL (PH8.5),100MM MGCL2 |