2W76
Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: FpvA-Pvd(Pa6)-Fe complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.422, 129.932, 140.877 |
Unit cell angles | 90.00, 130.77, 90.00 |
Refinement procedure
Resolution | 97.130 - 2.800 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o5p |
RMSD bond length | 0.019 |
RMSD bond angle | 1.985 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.370 |
Number of reflections | 59938 | |
<I/σ(I)> | 7.9 | 2.7 |
Completeness [%] | 92.5 | 83.5 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |