2W4I
Crystal structure of Helicobacter pylori glutamate racemase in complex with D-Glutamate and an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.074, 93.982, 101.295 |
Unit cell angles | 90.00, 91.46, 90.00 |
Refinement procedure
Resolution | 24.670 - 1.870 |
R-factor | 0.158 |
Rwork | 0.156 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jfz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.352 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.800 | 1.970 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.060 | 0.200 |
Number of reflections | 210077 | |
<I/σ(I)> | 9 | 3.8 |
Completeness [%] | 97.0 | 90.3 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100MM TRIS PH 8, NAAC 150 MM, 15% GLYCEROL, PEG 3350 20-35% |