2VWJ
Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 100.700, 161.910, 221.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.730 - 2.780 |
R-factor | 0.229 |
Rwork | 0.227 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tgo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.407 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.100 | 0.440 |
Number of reflections | 45193 | |
<I/σ(I)> | 11.1 | 2.2 |
Completeness [%] | 98.7 | 98.9 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.3 | 0.2M TRIPOTASSIUM CITRATE PH8.3, 20% PEG 3350 |