2VQC
Structure of a DNA binding winged-helix protein, F-112, from Sulfolobus Spindle-shaped Virus 1.
Replaces: 2CMXExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.891940, 0.979180, 0.979320 |
Spacegroup name | H 3 |
Unit cell lengths | 87.716, 87.716, 34.491 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.530 - 2.300 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.19900 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 0.923 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.110 |
Number of reflections | 4411 | |
<I/σ(I)> | 20.6 | 12.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.7 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | HANGING DROP VAPOR DIFFUSION IN 0.1M MES (PH 6.0). QIUCK SOAK IN ML CONTAINING 25% GLYCEROL AS A CRYOPROTECTANT. |