2VIF
Crystal structure of SOCS6 SH2 domain in complex with a c-KIT phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.377, 60.143, 71.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.690 - 1.450 |
| R-factor | 0.142 |
| Rwork | 0.139 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.475 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.030 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.089 | 0.704 |
| Number of reflections | 23956 | |
| <I/σ(I)> | 10.7 | 3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2M AMMONIUM SULPHATE, 30% PEG5000 MONOMETHYLETHER, 0.1M MES PH6.5 |
