Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.746, 121.746, 258.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.670 - 2.600 |
| R-factor | 0.26 * |
| Rwork | 0.261 |
| R-free | 0.33000 * |
| Structure solution method | MIR |
| RMSD bond length | 0.028 * |
| RMSD bond angle | 1.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.101 * | 0.537 * |
| Number of reflections | 66642 | |
| <I/σ(I)> | 5.0515 | 1.16 |
| Completeness [%] | 99.4 | 99.4 * |
| Redundancy | 5.6 * | 3.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 | 16 * | 18% PEG, 10MM NA/K PHOSPHATE PH 6.2, 200MM NACL, 4MM DTT, IP6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG1000 | 18 (%) | |
| 3 | 1 | reservoir | sodium potassium phosphate | 100 (mM) | pH6.2 |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 4 (mM) |
