2UVR
Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-08-08 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 94.719, 104.197, 52.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.070 - 2.900 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c22 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.080 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.140 | 0.610 |
Number of reflections | 11732 | |
<I/σ(I)> | 8.72 | 1.8 |
Completeness [%] | 98.3 | 89 |
Redundancy | 4.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 8% PEG 3350, 10 MM TRIS-HCL PH 7.5, 50 MM CA(CH3CO2)2, 50MM NACL, 3.5% GLYCEROL |