Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 285 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-02 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.800, 79.700, 60.800 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT AND MOLECULAR REPLACEMENT AVERAGING BETWEEN TWO CRYSTAL FORMS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.500 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.047 | |
| Total number of observations | 19983 * | |
| Number of reflections | 7850 | |
| Completeness [%] | 77.9 | 23.2 * |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.3 | 6-8%(W/V) PEG 8000, 300 MM TRIS, PH8.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-4 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 3-4 (%(w/v)) | |
| 3 | 1 | drop | Tris-HCl | 25-150 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 6-8 (%(w/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 50-300 (mM) |
