2SAK
STAPHYLOKINASE (SAKSTAR VARIANT)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.600, 43.730, 54.270 |
| Unit cell angles | 90.00, 115.63, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 * | |
| Total number of observations | 76483 * | |
| Number of reflections | 11949 | |
| <I/σ(I)> | 25 | 8.5 |
| Completeness [%] | 95.8 | 100 |
| Redundancy | 6.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | Rabijins, A., (1997) Protein.Struct.Funct.Genet., 27, 160. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.1 (M) | ||
| 2 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 3 | 1 | drop | PEG4000 | 17.5 (%) | |
| 4 | 1 | drop | protein | 17.5 (mg/ml) | |
| 5 | 1 | drop | HEPES | 25 (mM) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | PEG4000 | 35 (%) |
