2RH5
Structure of Apo Adenylate Kinase from Aquifex Aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.681, 157.596, 84.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.140 - 2.480 |
R-factor | 0.2 |
Rwork | 0.197 |
R-free | 0.25500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.268 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.480 | 2.500 |
Rmerge | 0.076 | 0.885 |
Number of reflections | 24570 | |
<I/σ(I)> | 9.9 | |
Completeness [%] | 96.4 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 28-30% (w/v) PEG 3000, 200 mM sodium acetate, and 100 mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 298K |