2RF7
Crystal structure of the escherichia coli nrfa mutant Q263E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.992 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.292, 91.201, 295.486 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.310 - 2.040 |
R-factor | 0.17411 |
Rwork | 0.171 |
R-free | 0.22642 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rdz |
RMSD bond length | 0.018 |
RMSD bond angle | 1.499 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.743 | 2.150 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.096 | 0.327 |
Number of reflections | 137863 | |
<I/σ(I)> | 3.6 | 0.9 |
Completeness [%] | 97.1 | 92.9 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES pH 7.5, 20 % PEG 10 K, VAPOR DIFFUSION, HANGING DROP, temperature 277K |