2RDI
Snapshots of a Y-family DNA polymerase in replication: Dpo4 in apo and binary/ternary complex forms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.927, 51.069, 86.026 |
Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
Resolution | 20.350 - 1.920 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24700 |
Starting model (for MR) | 1jx4 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.427 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.043 | 0.507 |
Number of reflections | 28887 | |
<I/σ(I)> | 16.8 | |
Completeness [%] | 93.8 | 90.6 |
Redundancy | 3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 294 | 20% PEG3350, 0.2M lithium nitrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |