2QSG
Crystal structure of Rad4-Rad23 bound to a UV-damaged DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Detector technology | CCD |
Collection date | 2006-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97922 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 79.644, 79.644, 403.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.100 |
R-factor | 0.248 |
Rwork | 0.246 |
R-free | 0.27700 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.241 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 23795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM bis-tris propane, 100mM sodium chloride, 6% (v/v) isopropanol, 14 mM calcium chloride and 5 mM dithiothreitol, pH 6.8, hanging-drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | bis-tris propane | ||
10 | 1 | 2 | dithiothreitol | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | isopropanol | ||
5 | 1 | 1 | dithiothreitol | ||
6 | 1 | 2 | bis-tris propane | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | CaCl2 | ||
9 | 1 | 2 | isopropanol |