2QOJ
Coevolution of a homing endonuclease and its host target sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-12-09 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.353, 72.701, 61.100 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 29.300 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.753 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.035 | 0.140 |
| Number of reflections | 20093 | |
| <I/σ(I)> | 18.4 | 5.7 |
| Completeness [%] | 92.1 | 76.8 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5 |
