2QKT
Crystal Structure of the 5th PDZ domain of InaD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-30 |
Detector | MAC Science DIP-2030 |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 95.740, 95.740, 48.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
Rwork | 0.224 |
R-free | 0.24200 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 14738 | |
<I/σ(I)> | 0.443 | 16.7 |
Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |