2QJZ
Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9798, 1.1271, 0.7999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.334, 65.220, 63.091 |
Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
Resolution | 45.220 - 1.250 |
R-factor | 0.177 |
Rwork | 0.174 |
R-free | 0.19800 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.054 | 0.321 |
Number of reflections | 63420 | |
<I/σ(I)> | 13.8 | 3.2 |
Completeness [%] | 97.4 | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |